º» ½Ã¼³¿¡¼´Â¼¼°¡Áö Á¾·ùÀÇpeptide library¸¦ Á¦°øÇÑ´Ù.°¢ library´Â Ž»ö´ë»óÀǼº°Ý¿¡ µû¶óÀ̿뵵°¡ ´Ù¸£´Ù.µû¶ó¼ ¼¼°¡ÁöÀÇlibrary¿¡ ´ëÇØ¼ °£´ÜÈ÷¼³¸íÇÑ´Ù. ¿¬±¸Àڴ°¢ libraryÀÇ ¼º°Ý°úÀÌ¿ëµÈ ¹®ÇåÀ»ÂüÁ¶ÇÑ ÈÄ ÇÊ¿äÇÑlibrary¸¦ ¼±ÅÃÇϱâ¹Ù¶õ´Ù.1.One Bead One Peptide Library(OBOPL)(1)
ÀÌ library´Â±×¸²1¿¡¼ ¼³¸íµÈ¹Ù¿Í °°ÀÌ synthesis, mix±×¸®°í divide¹æ¹ýÀ¸·Îresin¸¶´Ù ƯÀÌÇÑpeptide ¼¿ÀÌ ºÎÂøµÇµµ·Ï°í¾ÈµÇ¾ú´Ù.°¢ bead¸¶´Ù ¼¿°áÁ¤Çϱ⿡³Ë³ËÇÑ ¾çÀÇpeptide(100pmol)°¡ ºÎÂøµÇ¾îÀÖ´Ù. BeadµéÀ» petridish¿¡ ´ã°í ´ÜÀÏÇ×ü³ªºÐ¸®Á¤Á¦µÈ¼ö¿ëü ¹× È¿¼Ò¿Í¹ÝÀÀ½ÃŲ ÈÄÀÌµé ´Ü¹éÁúÀ̰áÇյǾî ÀÖ´Âbead¸¦ ã¾Æ ³»°ÔµÇ´Âµ¥ ±×¸²2¿¡¼¼³¸íµÇ¾úµíÀÌ´ÜÀÏÇ×üÀΰæ¿ì enzyme-liked secondary antibody¸¦ÀÌ¿ëÇÏ¿© ¹ß»öµÈbead¸¦ Çö¹Ì°æÇÏ¿¡¼Ã£À» ¼ö ÀÖ´Ù.±× ¿Ü¿¡ ¼ö¿ëü³ªÈ¿¼Òµµ ¹ß»ö½Ã½ºÅÛÀ» ÀÌ¿ëÇÏ¿©´Ü¹éÁúÀÌ ºÎÂøµÈbead¸¦ ãÀ» ¼öµµÀÖ´Ù. ±×·¯³ªpeptide°¡ resin¿¡ ºÎÂøµÇ¾îÀÖ¾î ligand¿Í ¼ö¿ëü°áÇÕÀ» ¾ïÁ¦Çϰųª¼¼Æ÷¿¡ ¿µÇâÀ»³¢Ä¡´Â peptideµîÀ»Å½»öÇÏ´Â system¿¡´Â»ç¿ëµÉ ¼ö°¡¾ø´Ù. Áö¿ø½Ã¼³¿¡¼Á¦°øÇÏ´Â OBOPLÀºpentapeptide sequence¿Í liker·Î ±¸¼ºµÇ¾îÀÖ°í, OBOPLÀÇ ÁúÀº¾Æ¹Ì³ë»êµéÀÌequimolarÇÏ°Ô ¿¬°áµÇ¾îÀÖÀ½°ú ´ÜÀÏÇ×ü¸¦»ç¿ëÇÏ¿© ¾Ë·ÁÁøepitopeÀÌ Ã£¾ÆÁüÀ¸·Î±Ô¸íÇÑ´Ù.
2.Positional Scanning SyntheticPeptide Combinatorial Library(PS-SPCL)(2)
PeptideÀÇ °¢À§Ä¡¸¦ À̹̾ƴ ¾Æ¹Ì³ë»êÀ¸·Î°íÁ¤½ÃŰ°í³ª¸ÓÁö À§Ä¡´Â20°³ÀÇ ¾Æ¹Ì³ë»êÈ¥ÇÕ¹°·Î ¿¬°á½ÃŰ´Â¹æ¹ýÀ¸·Î ±×¸²3¿¡¼¼³¸íµÇ¾úµíÀÌhexapeptide library°æ¿ì 120°³ÀÇpeptide poolÀÌ ¸¸µé¾îÁö°ÔµÈ´Ù. °¢ pool³»¿¡´Â3¹é¸¸°³ÀÇ ¼¿ÀÌÀÖ°Ô µÇ°í °¢ ¼¿Àdz󵵴 10nM±îÁö°¡´ÉÇÏ´Ù. ´ëºÎºÐÀÇȦ¸ó°ú ¼ö¿ëüÀÇbinding constant´Â 10-10 - 10-12MÀ̹ǷÎÀÌ library¸¦ ÀÌ¿ëÇÏ¿©È¦¸ó ¾ïÁ¦Á¦¸¦Ã£À» ¼ö ÀÖ´Ù.120°³ÀÇ poolÀ» testÇϸéhexapeptideÀÇ °¢ À§Ä¡¿¡¼Áß¿äÇÑ ¾Æ¹Ì³ë»êÀ»¾Ë¾Æ³¾ ¼ö ÀÖ´Ù.ÈçÈ÷ ¿©·¯ peptide¼¿µéÀÇcooperative effect¿¡ ÀÇÇÑ °á°úµµ³ªÅ¸³ª°Ô µÇ¹Ç·Î°¢ À§Ä¡¿¡¼ ¿©·¯°³ÀÇÁß¿äÇÑ ¾Æ¹Ì³ë»êÀ̳ªÅ¸³ª´Â °æ¿ì°¡ÀÖ´Ù. µû¶ó¼¾ò¾îÁø ¾Æ¹Ì³ë»êµéÀ»combinationÀ¸·Î ¿¬°áÇÏ¿©È¿À²ÀÌ ÀÖ´Âpeptide¼¿À» ã°ÔµÈ´Ù. ÀÌ·¯ÇÑlibrary¸¦ ÀÌ¿ë ÇÏ¿©Ç×±ÕÁ¦(3), Ç×»ýÁ¦(3),È¿¼Ò¾ïÁ¦Á¦(4),Ȧ¸ó antagonist(5)µîÀ̹߰ߵǾú´Ù.¼¼Æ÷³ª µ¿¹°À»´ë»óÀ¸·Î Ž»öÇÒ¼ö ÀÖ´Â ÀåÁ¡ÀÌÀÖ´Ù.
3. Phage-Displayed Peptide Library(6)
M13 phageÀÇ coat proteinpIII N-terminus¿¡ ÇØ´çÇÏ´ÂÀ¯ÀüÀÚ ºÎÀ§¿¡¹«ÀÛÀ§¼¿ÀÇoligonucleotide¸¦ »ðÀÔÇÏ¿©Æ¯ÀÌÇÑ ¼¿À̰¢ phage¿¡ ³ëÃâµÇµµ·ÏÇÏ´Â °ÍÀ¸·Îpanning¿¡ ÀÇÇÏ¿© ã¾ÆÁøphage cloneµéÀÇ DNA¼¿·ÎºÎÅÍ peptide¼¿À»°áÁ¤ÇÒ ¼ö ÀÖ´Ù.¿¬±¸´ë»óÀǴܹéÁú(¼ö¿ëüµî)°ú ³ôÀº ģȼºÀ»°¡Áø phageµéÀ» panning¿¡ÀÇÇÏ¿© ãÀ»¼ö ÀÖ´Â ÀåÁ¡ÀÌÀÖ´Ù. ´ÜÀÏ Ç×üepitope mappingµî¿¡ ÀÌ¿ëµÇ°íÀÖ´Ù. º» ½Ã¼³¿¡¼Á¦Á¶µÈ library´Â cyclicÇüŸ¦°¡Áø peptide(±×¸² 4 ÂüÁ¶)conformationÀ̰íÁ¤µÇ¾î flexibleÇÑpeptideµé º¸´Ù ´õ ģȼºÀ̳ôÀº peptide¸¦ ãÀ»¼ö ÀÖ´Â ÀåÁ¡ÀÌÀÖ´Ù.(7)
4. PeptideÇÕ¼º¹× ºÐ¼® ¼ºñ½º
Peptide libraryŽ»ö¿¬±¸´Â¼±º°µÈ peptideµéÀÇÇÕ¼º ¹× ºÐ¼®À̺´ÇàµÇ¹Ç·ÎÀÌ·¯ÇÑ ¼ºñ½ºµµº» ½Ã¼³¿¡¼Á¦°øÇÑ´Ù. ´ÜpeptideÀÇ ±æÀ̰¡ 10merÀÌ»óµÇ°Å³ªcyclic formÀÇ ÇÕ¼ºÀÌÇÊ¿äÇÑ °æ¿ì´Â¿Ü±¹ ȸ»ç¿¡ÇÕ¼ºÀ» ÀÇ·ÚÇϵµ·Ï±ÇÀåÇÑ´Ù. ±×µ¿ÇÑÁ¢ÃËÀÌ ÀÖ¾ú´øÈ¸»çµéÀ» ¼Ò°³ÇØÁÙ ¼ö ÀÖ´Ù.
5. Library °ü·ÃÁ¤º¸ Á¦°ø ¼ºñ½º
Library¿¡ °ü·ÃµÈ¹®ÇåÀ» ±¸ºñÇϰíÀÖÀ¸¹Ç·Î ¿øÇÏ´ÂÀڷḦ Á¦°øÇØÁÙ ¼ö ÀÖ´Ù.